Analysis Note

Positive ControlU-2 OS cells, HeLa NE cells, Human salivary gland tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.

This Anti-HTT Mouse mAb (3F1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of HTT.

Recognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)